Isolation and molecular identification of bacteria from sheep with eye infections

Abstract

Background: Ocular disease in sheep is a severe concern for the health and welfare of livestock animals, as well as losses of productivity and value to the livestock industry. Aim: This study aimed to isolate and characterize bacteria in sheep with eye disease on the molecular level. Methods: One hundred fifty sheep with eye infections were treated, and tissue samples were taken for microbiological studies. We isolated bacteria from traditional cultures and discovered molecules by polymerase chain reaction (PCR) of single bacterial genes. Results: A total of 150 ocular samples were collected from sheep, with bacterial growth observed in 120 samples, resulting in an isolation rate of 80%. Staphylococcus aureus was the most bacteria isolated in this study, which PCR also confirmed. We found antibiotic-resistant bacteria such as S. aureus, Escherichia coli, and Pasteurella multocida. These results reveal that preventing sheep ocular infections requires the effective use of antibiotics. Conclusion: This study suggests the prevalence of bacterial infection in sheep eyes and argues the utility of molecular methods in veterinary diagnosis. Record levels of antibiotic resistance must be maintained in animal husbandry and the use of antibiotic stewardship programs. Keywords: Sheep, Eye, Bacteria, PCR.