Effect of the injection of protoscolex antigens for hydatid cysts on some immunological parameters and the protection of injury
| dc.contributor.author | Hussain Abdalwahab Bdewi | |
| dc.contributor.author | Abbas Khetam Mohammed | |
| dc.contributor.author | Hamad Laheeb Rajab | |
| dc.date.accessioned | 2025-03-03T10:31:58Z | |
| dc.date.issued | 2024-11-11 | |
| dc.description | This study investigates the potential for preparing antigens from protoscolex found within hydatid cysts and evaluates the possibility of using these antigens to protect against damage caused by the cysts. Cystic echinococcosis, caused by the tapeworm Echinococcus granulosus, is a significant parasitic disease prevalent in Iraq and Eastern Mediterranean countries, with a global distribution. The protoscolex, which is a developmental stage of the parasite, is found inside hydatid cysts. These cysts are formed from the germinal layer of the cyst, and in this study, they were isolated from human patients undergoing surgery at Fallujah Teaching Hospital. The goal was to assess the impact of these antigens on both cellular and humoral immune responses. The study was conducted on 20 Balb/c white mice, which were divided into two groups: The first group was injected subcutaneously with 0.1 ml of the protoscolex antigen to evaluate its effects on antibody production (IgG, IgA, IgM, IgE), complement activation (C3, C4), neutrophil function, and phagocytosis. In the second group, protoscolex was injected into the liver of the mice at the same concentration to assess the antigen's ability to reduce or prevent liver injury caused by the parasite. The results showed a significant increase in all antibody types (IgG, IgA, IgM, IgE) and complements (C3, C4), as well as enhanced neutrophil activity, including an increase in phagocytosis and a reduction in the NBT dye reduction test at 60 minutes after injection. However, no significant differences were observed in the viability of neutrophil cells. Regarding the protection against injury, the antigen showed a significant effect, reducing injury by 56.4% compared to the control group. Additionally, the hydatid cysts in the experimental group were smaller in size compared to the control group, indicating that the antigen may help reduce the size and impact of these cysts. In conclusion, the study suggests that antigens prepared from protoscolex inside hydatid cysts can enhance immune responses and provide protection against the injury caused by hydatid cysts, showing potential for future therapeutic approaches. | |
| dc.description.abstract | Abstract This study aimed to that capability of preparation of antigens from protoscolex found inside the hydatid cyst and likewise possibility of preparing antigen providing protection of the injury of this cysts. Cystic echinococcosis is the more important parasitic disease caused by a cestode tapeworm of species Echinococcus granulosus, spreading a lot in Iraq and the Eastern Mediterranean Countries in addition to its world distribution. The protoscolex found inside hydatid cysts results from the germinal layer of hydatid cyst isolated from people by the surgery section at the Fallujah teaching hospital to find out the extent of its influence on both of the cellular and humeral immunity. 20 white mice (Balb/c) were divided into two groups, the first group of white mice (Balb/ c) was injected subcutaneously with a concentration of 0.1ml of antigen and it measured antibodies IgG, IgA, IgM, IgE as well as injected with complement C3, C4 and the insulation of neutrophil cells and measuring percentage of phagocytosis, reduction of a dye NBT and viability of neutrophil cells and in the second group, the protoscolex was injected in the liver with a concentration 0.1ml to find out the extent of prevention provided by the antigen to prevent or to reduce injury. The results showed a significant increase for all types of antibodies and complements C3 , C4 and reduction of a dye NBT and phagocytosis especially at the time 60 minute and the results were not showing significant differences on viability of neutrophil cells. As for prevention of injury, the results have shown a significant difference at the level < 0.05 and reached the percentage of prevention 56.4 % compared to the subordinate control. The results showed small size of the hydatid cyst in the experience parameters compared to the subordinate control. | |
| dc.description.sponsorship | self-finance | |
| dc.identifier.citation | Bdewi, H. A., Mohammed, A. K., & Rajab, H. L. (2024). Effect of the injection of protoscolex antigens for hydatid cysts on some immunological parameters and the protection of injury. Research Journal of Biotechnology Vol, 19, 11. | |
| dc.identifier.uri | https://worldresearchersassociations.com/Archives/RJBT/Vol(19)2024/November%202024/Effect%20of%20the%20injection%20of%20protoscolex%20antigens%20for%20hydatid.aspx | |
| dc.identifier.uri | https://ds.uofallujah.edu.iq/handle/123456789/462 | |
| dc.language.iso | en | |
| dc.publisher | Research Journal of Biotechnology | |
| dc.relation.ispartofseries | https://worldresearchersassociations.com/Archives/RJBT/Vol(19)2024/November%202024/Effect%20of%20the%20injection%20of%20protoscolex%20antigens%20for%20hydatid.aspx; https://worldresearchersassociations.com/Archives/RJBT/Vol(19)2024/November%202024/Effect%20of%20the%20injection%20of%20protoscolex%20antigens%20for%20hydatid.aspx | |
| dc.title | Effect of the injection of protoscolex antigens for hydatid cysts on some immunological parameters and the protection of injury | |
| dc.type | Article |